Bisphenol-A Exposure Causes Increased Oxidation of LDL and Its Abduct in Rats

ABSTRACT

Background and Objectives: Living organisms are exposed to oxidant agents constantly from both endogenous and exogenous sources. One such oxidant agent is Bisphenol-A (BPA) and its exposure is capable to modify biomolecules and induce damages. Bisphenol-A (BPA) is a contaminant with increasing exposure. It exerts toxic effects on cells. This study investigates the possibility of BPA exposure on Low-Density Lipoprotein (LDL) perturbations at prevailing low exposure doses in female albino Wistar rats, following exposure for three (3) month. Materials and Methods: Total 12 groups were formed; out of which 11 experimental groups, each containing 10 non-pregnant female rats were administered; 0.05, 0.1, 0.2, 0.3, 0.4, 0.5, 0.6, 0.7, 0.8, 0.9 and 1 mg of BPA kg^-1 b.wt.day^-1. The 12th control group was given water.  Blood was collected from animals at the end of every week of the study and serum sample specimens analyzed by routine diagnostic procedures for oxidized LDL such as Malondialdehyde modified-LDL (MDA-LDL), Oxidized Phospholipids-LDL (OX-PL LDL), N (epsilon) (carboxymethyl) lysine-modified-LDL (CML LDL) and 4-hydroxynonenal-LDL (HNE-LDL) using autochemical analyzer. Results: Significantly increased concentrations of serum oxidized LDL such as MDA-LDL, OX-PL LDL, CML LDL and HNE-LDL were observed at all concentrations of BPA exposure. Conclusion: Bisphenol-A alters oxidized LDL such as MDA-LDL, OX-PL LDL, CML LDL and HNE-LDL balance and causes disturbance of internal oxidative statues.