Research Article | Open Access

Influence of Growth Regulators on Micropropagation System of Medicinal Plant Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.)

    Waldemar Kiszczak

    Department of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Str., 96-100 Skierniewice, Poland

    Urszula Kowalska

    Department of Plant Anatomy and Cytology, Faculty of Biology, University of Warsaw, 1 Miecznikowa Str., 02-096 Warsaw, Poland

    Maria Burian

    Department of Plant Anatomy and Cytology, Faculty of Biology, University of Warsaw, 1 Miecznikowa Str., 02-096 Warsaw, Poland

    Sława Glinska

    Laboratory of Microscopic Imaging and Specialized Biological Techniques Faculty of Biology and Environmental Protection, University of Lodz, Banacha 12/16, 90-237 Lodz, Poland

    Krystyna Górecka

    Department of Applied Biology, Research Institute of Horticulture, Konstytucji 3 Maja 1/3 Str., 96-100 Skierniewice, Poland


Received
15 Jan, 2021
Accepted
20 Mar, 2021
Published
03 Jun, 2021

ABSTRACT

Background and Objective: Currently, an interest in the cultivation of yacon is growing, which is a plant with strong medical potential. Therefore, studies on the optimization of the yacon micropropagation method in tissue cultures were undertaken. Materials and Methods: Reproduction of this plant is conducted with the use of traditional methods by green seedlings or 8-12 cm long offsets (‘seeds’) taken from the underground and aboveground rootstock (‘crown’). The influence of different disinfection methods, various initial explants and growth regulators, also their concentrations on the efficiency of micropropagation protocol in conducted research was analyzed. Results: The most effective disinfection was the application of 70% ethanol and 0.5% Tween 20. The optimal initial explants in the current research were apical and lateral buds cultured on MS medium supplemented with 0.2 mg L-1 kinetin and 1 mg L-1 indole-3-acetic acid. The most intensive shoot regeneration occurred on MS media with the addition of 1 mg L-1thidiazuron and 1-naphthylacetic acid. Histological observations demonstrated the strong effect of these growth substances on shoots organogenesis from callus tissue. Conclusion: Shoots obtained from in vitro cultures rooted easily on modified MS media with ½ or ¼ concentration of macroelements, 20 g L-1 sucrose and 3 mg L-1 1-naphthylacetic acid and then adapted to ex vitro conditions in 100%.

How to Cite this paper?


APA-7 Style
Kiszczak, W., Kowalska, U., Burian, M., Glinska, S., Górecka, K. (2021). Influence of Growth Regulators on Micropropagation System of Medicinal Plant Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) . Asian Journal of Emerging Research, 3(1), 44-48. https://doi.org/10.3923/ajerpk.2021.44.48

ACS Style
Kiszczak, W.; Kowalska, U.; Burian, M.; Glinska, S.; Górecka, K. Influence of Growth Regulators on Micropropagation System of Medicinal Plant Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) . Asian J. Emerg. Res 2021, 3, 44-48. https://doi.org/10.3923/ajerpk.2021.44.48

AMA Style
Kiszczak W, Kowalska U, Burian M, Glinska S, Górecka K. Influence of Growth Regulators on Micropropagation System of Medicinal Plant Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) . Asian Journal of Emerging Research. 2021; 3(1): 44-48. https://doi.org/10.3923/ajerpk.2021.44.48

Chicago/Turabian Style
Kiszczak, Waldemar, Urszula Kowalska, Maria Burian, Sława Glinska, and Krystyna Górecka. 2021. "Influence of Growth Regulators on Micropropagation System of Medicinal Plant Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) " Asian Journal of Emerging Research 3, no. 1: 44-48. https://doi.org/10.3923/ajerpk.2021.44.48